Bacteriostatic Water 3mL – Preserved Sterile Diluent

Key Facts: Bacteriostatic Water

Product Overview

Bacteriostatic Water for Injection (bWFI) is a sterile, nonpyrogenic preparation of purified water containing 0.9% benzyl alcohol as a bacteriostatic preservative. This specialized diluent is designed for reconstituting lyophilized peptides and other research compounds, with the preservative enabling safe multi-dose vial access over extended periods.

Unlike preservative-free Sterile Water for Injection (which must be discarded after a single use), Bacteriostatic Water can be safely accessed multiple times over up to 28 days, as the benzyl alcohol continuously inhibits bacterial and fungal growth. This makes it the preferred diluent for research applications involving repeated dosing or extended experimental protocols.

BIONIX Bacteriostatic Water meets USP specifications for sterility and composition, providing researchers with a reliable, pharmaceutical-grade diluent for peptide research applications.

Specifications

Mechanism: How Benzyl Alcohol Preserves Sterility

Benzyl alcohol functions as a bacteriostatic agent, meaning it inhibits the growth and reproduction of bacteria without necessarily killing existing microorganisms.

Antimicrobial Action

Preservation Mechanism:

• Disrupts bacterial cell membrane integrity
• Interferes with microbial metabolic processes
• Prevents proliferation of contaminating organisms
• Maintains sterility despite multiple vial punctures

pH Stability

The slightly acidic to neutral pH range is compatible with the majority of research peptides and supports optimal benzyl alcohol preservation activity.

Packaging

Frequently Asked Questions

What is Bacteriostatic Water used for in peptide research?

Bacteriostatic Water is used to reconstitute (dissolve) lyophilized peptide powders into liquid solutions for research applications. The 0.9% benzyl alcohol preservative inhibits bacterial growth, allowing the solution to be used multiple times over up to 28 days. This makes it ideal for multi-dose research protocols where peptides are withdrawn from the same vial over an extended period.

How long can I use Bacteriostatic Water after opening?

Once opened (first needle puncture), Bacteriostatic Water should be used within 28 days when stored properly at room temperature or refrigerated. The benzyl alcohol preservative maintains sterility during this period by inhibiting bacterial and fungal growth. Always inspect the solution before use and discard if it appears cloudy, discolored, or contains particles.

What is the difference between Bacteriostatic Water and Sterile Water?

The key difference is the preservative. Bacteriostatic Water contains 0.9% benzyl alcohol which inhibits microbial growth, allowing multi-use access over 28 days. Sterile Water for Injection contains no preservative and must be used immediately after opening, with any remainder discarded. For research protocols involving repeated peptide dosing, Bacteriostatic Water is the preferred choice.

Can Bacteriostatic Water be frozen?

While unopened Bacteriostatic Water can be stored at room temperature or refrigerated (not frozen), reconstituted peptide solutions can be aliquoted and frozen at -20°C for extended storage. However, avoid repeated freeze-thaw cycles of reconstituted peptides as this can degrade peptide structure. Bacteriostatic Water itself should not be frozen.